A Culture Medium from Groundnut Meal. Part II

In a recent paper (Basil, Sen and Sen Gupta, 1945) we have shown that groundnut meal affords a culture medium for the growth of various intestinal organisms when it is digested with some proteolytic enzyme. The hydrolysate when further admixed with a trace of liver extract gives still better results. The latter observation led us to work on the growth of other delicate organisms, and the experimental data obtained are recorded in this paper. The broth.?A solvent-extracted meal as obtained by the process described by Basu and Sen Gupta (1944) was digested by papain in the presence of sodium thiosulphate according to the method previously described by us (Basu, Sen and Sen Gupta, 1945), and incorporated with 2 per cent as well as 5 per cent liver digest. The pH was adjusted to 7.8, and the oxidizable matter was 0.75 per cent. Bacteriological tests.?The following organisms were used to study the growth-promoting power of the broth: pneumococcus types I, II and III ; influenza bacillus ; gonococcus ; Streptococcus hcemolyticus and meningococcus. The results are recorded in the table. In working with the bazaar meal we thought that the processing during the extraction of groundnut oil by hydraulic press or the expressor process might destroy and|or produce deterioration in the nutrient substances of the nut, and that the broth obtained from it might not be suitable for the growth of the